An Atlas of Preimplantation Genetic Diagnosis: An by Yury Verlinsky, Anver Kuliev

By Yury Verlinsky, Anver Kuliev

Largely illustrated, this atlas is a guide for the institution and attention of PGD in the framework of assisted replica and genetics providers. totally revised and up to date, the atlas comprises descriptions of the authors' pioneering paintings on polar physique dependent PGD for genetic and chromosomal problems. The authors' novel adventure of PGD for late-onset issues with genetic predisposition may be of unique curiosity. Their collected PGD adventure for poor-prognosis IVF sufferers offers additional facts of the advance of scientific final result and the requirement for meiotic errors checking out for the next accuracy of preselecting aneuploidy-free embryos for move.

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Additional info for An Atlas of Preimplantation Genetic Diagnosis: An Illustrated Textbook & Reference for Clinicians, Second Edition

Sample text

6) After 3-5 min, the blastomere is aspirated into the pipette and transferred to a microscope slide with a small amount of hypotonic solu­ tion. (7) Under the control o f the inverted microscope with phase-contrast optics, the blastomere is constantly observed until drying is almost complete. Just at the time of drying (crystal­ lization) o f the hypotonic solution, the fixative is aspirated into the pipette and dropped onto the blastomere. While constantly observing the cell, just before drying occurs, fixative is dropped again.

Three-dimentional partial zona dissection for preimplantation genetic diagnosis and assisted hatch­ ing. Fertil Steril 1999;71:308-13 8. Verlinsky Y, Milayeva S, Evsikov S, et al. Preconception and preimplantation diagnosis for cystic fibrosis. Prenat Diagn 1992;12:103-10 9. Kaplan B, Wolf G, Kovalinskaya L, Verlinsky Y, Viability of embryos following second polar body removal in a mouse model. J Assist Reprod Genet 1995;12:747-9. 10. Strom C, Levin R, Strom S, et al. Neonatal outcome of preimplantation genetic diagnosis by polar body removal: the first 109 infants.

J Assist Reprod Genet 1995;12:747-9. 10. Strom C, Levin R, Strom S, et al. Neonatal outcome of preimplantation genetic diagnosis by polar body removal: the first 109 infants. Pediatrics 2000; 106: 650-3 I 1. Hardy J, Martin KL, Leese HJ, Winston RM, Handyside AH. Human preimplantation develop­ ment in vitro is not adversely affected by biopsy at the 8-cell stage. Hum Reprod 1990;5:708-14 12. Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte.

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